Anti-gammaH2AX (phospho-Ser139) Rabbit (Clone RM224)

Histone H2AX, a variant of the nucleosome core histone H2A, undergoes phosphorylation on serine 139 in response to DNA damage, particularly when the damage involves formation of double-strand breaks. Phosphorylated H2AX is also termed gammaH2AX. Induction of gammaH2AX in cells exposed to genotoxic agents is considered a sensitive and specific reporter of DNA damage. This antibody reacts to gammaH2AX when phosphorylated at Ser139 and does not detect other phosphorylated histones.Immunogen: A phospho-peptide corresponding to gammaH2AX (phospho-Ser139). Clone Designation: RM224. Formulation: (Request formulation change), 100 µg protein A affinity-purified antibody from an animal origin-free culture supernatant. Storage Buffer: 50% glycerol/PBS with 1% BSA and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, Multiplex, and WB. Cross Reactivity: (+) Phospho-gammaH2AXSer139, (-) Other phosphorylated histones.