Anti-GABAA Receptor beta3 Subunit

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS. They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: alpha1-6, beta1-4, gamma1-3, delta, epsilon, pi, theta, and rho1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by alpha1beta2gamma2 subunit-containing GABAA receptors, the major isoform found in the brain. The beta subunit of GABAA receptors interfaces with an alpha subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the gamma subunit. beta3 subunit-containing GABAA receptors are widely expressed in the cerebral cortex, cerebellum, olfactory bulb, and hippocampus. Phosphorylation of the beta3 subunit by PKA or PKC inhibits binding of the beta3 subunit with the clathrin adaptor protein AP2 and reduces GABAA receptor endocytosis. Mutations in GABRB3, which encodes the beta3 subunit isoform, have been found in patients with childhood absence epilepsy (CAE), infantile spasms (IS), and Lennox-Gastaut syndrome (LGS). Cayman's GABAA Receptor beta3 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor beta3 subunit at approximately 53 kDa from rat and mouse samples.Synonyms: GABRB3, Gamma-aminobutyric Acid Receptor Subunit beta3. Immunogen: Fusion protein from the cytoplasmic loop of the beta3 subunit of the rat GABAA receptor. MW: ~53 kDa. Formulation: (Request formulation change), 100 µl of Affinity-purified rabbit polyclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC, WB.