Anti-Fibroblast Growth Factor Binding Protein (FGFBP, FGF-BP, 17kD Heparin-Binding Growth Factor Bin

FGF-BP, also known as 17kD heparin-binding growth factor binding protein (HBp17) is a secreted, low affinity heparin binding protein. It binds reversibly to FGF-1, -2, -7, -10, and -22 and functions to release the FGFs from the extracellular matrix for interactions with FGF receptors. Human FGF-BP shares 66% amino acid sequence homology with the mouse protein. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. , ELISA (Direct): 0.5-1ug/ml. The detection limit is ~0.5ng/well. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.