Anti-ERK1 (Extracellular Signal-regulated Kinase 1, ERK-1, Esrk1, ERT2, Insulin-stimulated MAP2 Kina

Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on speci?c threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identi?ed and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identi?ed including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK (4) , c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (5) . MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (6,7,8) . Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml , Immunoprecipitation (11) : 2-5ug , Western Blotting (9,10) : 1ug/ml , , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.