Anti-ErbB3 (ERBB3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) , HGNC:3431, Er

Alternate Name: ERBB3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) , HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 , erbB-3, Domain Information: , Gene Ontology Terms: , Application(s): Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150kD band observed in Human lysates of T cell line JURKAT (calculated MW of 148kD according to NP_001973.2). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?, 0.1ug/ml staining of Jurkat lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See E3451-35L-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.