Anti-Dopamine Receptor, D2L, D2S

The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Two isoforms are reported for the D2 Dopamine Receptor, isoform 1 and isoform 2 which are expressed in the pituitary and brain, Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 0.5-1.0ug/ml, Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates. , Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms. , Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml., Optimal dilutions to be determined by the researcher. Positive Control: Monkey brain tissue, rat brain lysate, Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb, I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.