Anti-CD80 (B7-1 Costimulator Protein), Phycoerythrin conjugated, clone 5K18

CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. 1-4 The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. This antibody locks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. 2, 7, Applications: Suitable for use in Flow Cytometry and Immunoprecipitation and in vivo and in vitro blocking of ligand binding. Other applications have not been tested. Recommended Dilution: Flow Cytometry: < 0.2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.