Anti-CD8 alpha (LY-2), clone 6A242

Applications: , Suitable for use in Flow Cytometry, in vitro blocking studies, and Immunohistochemistry (frozen, paraffin-embedded following PLP Fixation (periodate-lysine paraformaldehyde). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100 Use 10ul to label 10e6 cells in 100ul., Immunohistochemistry: Does not require protein digestion pre-treatment of paraffin embedded sections, Does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections., Optimal dilution determined by the researcher, Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) , IgG Goat anti mouse IgG:RPE (rat adsorbed) , Recommended Negative Controls: Mouse IgG1 Negative Control , Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.