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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C2392-02B.100 | 100 µg | - | - |
3 - 19 business days* |
621.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B... more
Product information "Anti-CD40 (Biotin)"
Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1). The CD40-CD154 interaction appears to have a role in artherosclerosis and cardiovascular disorders (3,4,5). Applications: Suitable for use in Flow Cytometry, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml. 80ul labels 5x10e5 cells., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2392-02B at a concentration of 10ug/ml. , 2. Cells were washed twice and incubated with 2º reagent Streptavidin/R-Phycoerythrin (S7973-88P), after which they were washed three times, fixed and analyzed by FACS. , 3. Cells stained positive with a mean shift of 2.37 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. , 4. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD40 antibody (C2392-02A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | C2392-02B |
Properties
Application: | ELISA, FC, IP |
Antibody Type: | Monoclonal |
Clone: | 7H162 |
Conjugate: | Biotin |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | Murine L cells transfectants expressing human CD40 |
Format: | Affinity Purified |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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