Anti-CD274 (CD274 Molecule, B7 Homolog 1, B7H1, B7-H1, B7-H, Programmed Cell Death 1 Ligand 1, PDCD1

B7-H1, also known as PD-L1 and CD274, is a 50kD type I transmembrane protein belonging to the B7 family. B7-H1 contains one Ig-like C2-type domain and one Ig-like V-type domain. It is constitutively expressed in several tissues and is up-regulated on various tumors and activated dendritic cells, macrophages, and B cells. B7-H1 interacts with PD-1 on T cells to limit T cell activation and regulate immune tolerance. Within the extracellular region, mouse and human B7-H1 share 73% amino acid sequence identity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul, 1x10e6 cells, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from Balb/c mice. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.