Anti-CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029

CD274, also known as B7-H1 and PD-1L, is a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions, inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1, PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation., Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:10, 10ul labels 1x10e6 cells in 100ul., Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control, Hybridoma: Cells from immunized mice were fused with cells of the P3U1 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.