Anti-CD274 (B7-h1 Protein, B7-H1, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1)

B7-H1, also known as PD-L1 and CD274, is a 50kD type I transmembrane protein in the B7 family. B7-H1 contains one Ig-like C2-type domain and one Ig-like V-type domain. It is constitutively expressed in several tissues and is upregulated on various tumors and activated dendritic cells, macrophages, and B cells. B7-H1 interacts with PD-1 on T cells to limit T cell activation and regulate immune tolerance. Within the extracellular region, mouse and human B7-H1 share 73% aa sequence identity. Applications: Suitable for use in Direct ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 Jurkat human acute T cell leukemia cell line., Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human colon, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.