Anti-CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

CD106, otherwise known as VCAM-1 (vascular adhesion molecule 1), a 110kD inducible type I transmembrane glycoprotein and member of the immunoglobulin supergene family, which is predominantly expressed on vascular endothelium, and also on bone marrow stromal cells, follicular dendritic cells and some macrophages. CD106 interacts with the lymphocyte homing receptor VLA-4 (alpha4 beta1 integrin) and LPAM-1 (alpha4 beta7 integrin) and mediates leukocyte-endothelial cell adhesion and signal transduction. Expression of CD106 is upregulated following cytokine activation, and endothelial CD106 plays a role in the extravasation of leukocytes from blood vessels during inflammation. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:25. 10ul labels 1x10e6 cells in 100ul., Immunohistochemistry: Frozen sections, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-14C: IgG, Fc (HRP) Pab Gt xMo, I1904-19C: IgG, F(ab')2 (HRP) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.