Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C2446-01H.100 | 100 µg | - | - |
3 - 19 business days* |
916.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
CD104 (also GP150 and Integrin beta 4) is a 150-200kD member of the integrin beta family. It... more
Product information "Anti-CD104 (Integrin beta4)"
CD104 (also GP150 and Integrin beta 4) is a 150-200kD member of the integrin beta family. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683aa extracellular domain (ECD) (aa28-710) and a large 1089aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84aa substitution for the 84aa between aa21-704. A second shows a 144aa substitution for the first 704aa. Human beta 4 ECD shares 88% aa sequence identity with mouse beta4 ECD. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml, Using a colorimetric detection system, the detection limit for recombinant human Integrin 4 is ~25ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold., Flow Cytometry: 0.2mg/ml, 10ul labels 1-2.5x10e5 human A431 cells in a total reaction volume <200ul. , Immunocytochemistry: 10ug/ml detects CD104 in A431 human epidermoid carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C2446-01H overnight at 4ºC followed by anti-mouse IgG (FITC) at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-GP150, Anti-ITGB4, Anti-CD104, Anti-Integrin beta-4 |
Supplier: | United States Biological |
Supplier-Nr: | C2446-01H |
Properties
Application: | ELISA, FC, ICC, WB |
Antibody Type: | Monoclonal |
Clone: | 7H253 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | CHO-derived recombinant human Integrin Beta 4 (aa28-710, Accession # P16144) |
Format: | Affinity Purified |
Database Information
KEGG ID : | K06525 | Matching products |
UniProt ID : | P16144 | Matching products |
Gene ID : | GeneID 3691 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
-30 %
Discount Promotion
-30 %
Discount Promotion
Viewed