Anti-Caspase-11, clone 6D225

Caspase-11, (also known as CASP-11, ICH-3, ICE-B) promotes IL-b processing by procaspase-1 (ICE) and therefore may also have a role in inflammatory response as evident by a 30 fold increase in CASP-11 activity by LPS. The mouse and rat CASP-11 (373aa) are most homologous to human CASP-4 (377aa) protein (ICErel-II, TX, ICH-2) and could possibly be the ortholog of human CASP-4. The pro-or zymogen form of CASP-11 is activated by Granzyme-B or cathepsin-B and the processed enzyme consists of two large p20 (20kD) and two small ~p10 (10kDa subunits. CASP-11 is highly expressed in lung and spleen, weaker in heart and very little in liver, kidney, testis and skeletal muscle but has not been detected in the brain. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detects two bands of ~38kD and ~43kD., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.