Anti-c-erbB2 (HER-2, neu Receptor), clone 3F30

c-erbB-2, second member (c-erbB-2/HER-2/neu) of the c-erbB family is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein , Applications: Suitable for use in Tumor Growth Inhibition, Inhibition of Cell Proliferation, Electron Microscopy, Immunofluorescence, and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Tumor Growth Inhibition (~90%), Immunohistochemistry: Frozen only., Optimal dilutions to be determined by the researcher. Positive Control: SKBR-3 cells, or breast carcinomas , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ,