Anti-AXIN1 (Axin 1, Axin-1, AXIN, Axis Inhibition Protein 1, hAxin, MGC52315)

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), b-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of b-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: SK-N-SH Cell Lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.