Anti-AMP Activated Protein Kinase alpha1 (AMPKa1)

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kD) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detected AMP-activated Protein Kinase a1 in RIPA lysates from human non-stimulated A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1475-01. Proteins were visualized using an IgG (HRP) goat and a chemiluminescence detection system., Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer, Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb, I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.