Anti-ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidon

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml., Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue., Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.