Anti-ALK (CD246, Anaplastic Lymphoma Kinas, Ki-1, Tcrz)

ALK (Anaplastic Lymphoma Kinase, also CD246) is a 200­220kD member of the Insulin receptor subfamily, tyrosine kinase family, protein kinase superfamily of proteins. It shows restricted expression, being limited to select sympathetic and sensory neurons, endothelial cells and tumor cells. Although activation of ALK appears to induce cell proliferation, the exact ligand(s) for ALK is unknown. In a temporally­regulated context, midkine has been suggested to bind to ALK, and ionic Zn has been reported to activate ALK cytoplasmically. Mature human ALK is a 1602 amino acid (aa) type I transmembrane glycoprotein (SwissProt #:Q9UM73). It contains a 1020aa extracellular region (aa19­1038) that contains one protein­protein MAM domain (aa264­427), an LDL receptor class A region (aa437­473), a second MAM domain (aa480­631) and one utilized phosphorylation site at aa211. This is accompanied by a long 561 aa cytoplasmic domain that possesses a protein kinase domain (aa1116­1392) plus eleven utilized Tyr phosphorylation sites. There is one potential splice variant that contains a four aa insert after aa 549. There is also an 80kD soluble product that arises from cleavage of the extracellular domain. Although a third 140kD product is frequently seen in SDS­Page, its structural basis is unclear. Almost 20 distinct ALK fusion products arise from gene translocations involving multiple intracellular molecules. These seem to involve blocks of aa starting between aa1050­1060 and continuing to aa1620. Over aa19­1038, human ALK shares 88% aa sequence identity with mouse ALK. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 5­15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.