Anti-Ah Receptor (Aryl Hydrocarbon Receptor, AHR, Aromatic Hydrocarbon Receptor, BHLHE76, Class E Ba

AHR is a 100-105kD member of the bHLH/PAS transcription factor family. It is widely expressed and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes. Mouse AHR precursor is 848aa in length. It contains a nine aa prosegment, plus an 839aa mature molecule that contains a DNA binding motif (aa12-39), a bHLH region (aa40-80), two PAS domains (aa116-336) and one PAC segment that stabilizes the PAS domains (aa342-383). There are multiple alleles for mouse AHR. One 95-97kD allele shows a premature truncation after Ser805, while a second 112kD allele shows a 41aa substitution for aa843-848. Over aa706-805, mouse AHR shares 87-63aa identity with rat and human AHR, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.