Trypsin, 3X, Bovine Pancreas, TPCK-Treated, Sterile, Irradiated

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation. , Activity:, ~180 units per mg protein. , Protein: As reported, Chymotrypsin: As reported, Virus Testing: Mycoplasmsa, BVD, Parvovirus, Adenovirus, Rabies, Blue Tongue, Respiratory Synctial, Reovirus. Unit Definition: 1 unit hydrolyzes 1umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25ºC, pH 8.2, in the presence of 0.01M calcium ion. , Quality Control: SDS-PAGE, Certificate of Origin: Trypsin is derived from animal products of North American origin, collected in USDA-approved facilities and inspected to be free of disease., , Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or media. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.