Trypsin, 2X, Bovine Pancreas, Sterile, Irradiated

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Quality Control: SDS-PAGE, Activity: >180u/mg Protein, (1mg trypsin > 180 TAME units, 10,350 BAEE units, 3450 USP/NF units), Unit Definition: 1 unit of trypsin hydrolyzes 1umole of p-toluenesulfonyl-L-arginine methyl ester (TAME) per minute @ 25ºC, pH 8.2, in the presence of 0.01M calcium ion. Virus Testing: None Detected Bovine Mycoplasma, Bovine Adenovirus (BAV), Rabies, Bovine Blue Tongue (BTV), Reovirus (REO), Bovine Parvovirus (BPV), Bovine Resp. Synctial (BRSV), Bovine Diarrhea virus. Appliations: Sutiable for use in Protein Sequencing, Cell and Tissue Disassociation. Other applications not tested. Recommended Dilutions: Monolayer Dissociation: 0.002-0.01% depending upon strain., Primary Embryo Tissues: 0.01-0.1%, Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute to nominal volume,aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: North America , Certificate of Origin, The raw animal product used in the manufacturing of Trypsin is derived from materials originating in North America. Material is processed in a USDA inspected facility. Note: Trypsin is subject to a pH <3.0 for >5 hours during processing.