TAQuest(TM) qPCR Master Mix for TaqMan Probes *No ROX*

TAQuest(TM) qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. The master mix provides all of the essential components including our proprietary TAQuest(TM) Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer, except the template, primers and probes. The Hot Start Taq DNA polymerase allows you to set up a PCR reaction at room temperature, thus minimizing non-specific product formation. The optimized composition ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. TAQuest(TM) qPCR Master Mix for TaqMan Probes has been designed to be used for duplex reactions using internal positive controls. This master mix does not contain a ROX reference dye.