Serine protease inhibitor A3K (Serpina3k), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 22-418aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: FPDGTKEMDI VFHEHQDNGT QDDSLTLASV NTDFAFSLYK KLALKNPDTN IVFSPLSISA ALALVSLGAK GKTMEEILEG LKFNLTETPE ADIHQGFGNL LQSLSQPEDQ DQINIGNAMF IEKDLQILAE FHEKTRALYQ TEAFTADFQQ PTEAKNLIND YVSNQTQGMI KELISELDER TLMVLVNYIY FKGKWKISFD PQDTFESEFY LDEKRSVKVP MMKMKLLTTR HFRDEELSCS VLELKYTGNA SALLILPDQG RMQQVEASLQ PETLRKWRKT LFPSQIEELN LPKFSIASNY RLEEDVLPEM GIKEVFTEQA DLSGITETKK LSVSQVVHKA VLDVAETGTE AAAATGVIGG IRKAILPAVH FNRPFLFVIY HTSAQSILFM AKVNNPK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Capsid proteins VP0, VP2, VP3 form a closed capsid enclosing the viral positive strand RNA genome. Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis., [Protein 2A]: Is not a protease., [Protein 2B]: Affects membrane integrity and cause an increase in membrane permeability., [Protein 2C]: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities., Protein 3A, via its hydrophobic domain, serves as membrane anchor., [Protease 3C]: Cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease., RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals. Reference: "Entry of human parechovirus 1." Joki-Korpela P., Marjomaki V., Krogerus C., Heino J., Hyypia T. J. Virol. 75:1958-1967(2001). Function: nan