Plasminogen activator inhibitor 1 (Serpine1), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 24-402aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: SPLPESHTAQ QATNFGVKVF QHVVQASKDR NVVFSPYGVS SVLAMLQLTT AGKTRQQIQD AMGFNISERG TAPALRKLSK ELMGSWNKNE ISTADAIFVQ RDLELVQGFM PHFFKLFRTT VKQVDFSEVE RARFIINDWV ERHTKGMISD LLAKGAVNEL TRLVLVNALY FNGQWKTPFL EASTHQRLFH KSDGSTISVP MMAQNNKFNY TEFTTPDGHE YDILELPYHG ETLSMFIAAP FEKDVPLSAI TNILDAELIR QWKSNMTRLP RLLILPKFSL ETEVDLRGPL EKLGMTDIFS STQADFTSLS DQEQLSVAQA LQKVKIEVNE SGTVASSSTA ILVSARMAPT EMVLDRSFLF VVRHNPTETI LFMGQLMEP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis . Reference: Isolation and characterization of the rat plasminogen activator inhibitor-1 gene.Bruzdzinski C.J., Riordan-Johnson M., Nordby E.C., Suter S.M., Gelehrter T.D.J. Biol. Chem. 265:2078-2085(1990). Function: Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).