NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (NDUFB10), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-172aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MPDSWDKDVY PEPPRRTPVQ PNPIVYMMKA FDLIVDRPVT LVREFIERQH AKNRYYYYHR QYRRVPDITE CKEEDIMCMY EAEMQWKRDY KVDQEIINIM QDRLKACQQR EGQNYQQNCI KEVEQFTQVA KAYQDRYQDL GAYSSARKCL AKQRQRMLQE RKAAKEAAAA TS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Reference: "One subunit of human NADH-ubiquinone oxidoreductase, hPDSW, located at 16p13.3 and down-regulated in a prostate cell line." Wang L., Zhang J., Smith D.I. Submitted (AUG-1998). Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.