DNA replication licensing factor MCM2 (MCM2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-904aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AESSESFTMA SSPAQRRRGN DPLTSSPGRS SRRTDALTSS PGRDLPPFED ESEGLLGTEG PLEEEEDGEE LIGDGMERDY RAIPELDAYE AEGLALDDED VEELTASQRE AAERAMRQRD REAGRGLGRM RRGLLYDSDE EDEERPARKR RQVERATEDG EEDEEMIESI ENLEDLKGHS VREWVSMAGP RLEIHHRFKN FLRTHVDSHG HNVFKERISD MCKENRESLV VNYEDLAARE HVLAYFLPEA PAELLQIFDE AALEVVLAMY PKYDRITNHI HVRISHLPLV EELRSLRQLH LNQLIRTSGV VTSCTGVLPQ LSMVKYNCNK CNFVLGPFCQ SQNQEVKPGS CPECQSAGPF EVNMEETIYQ NYQRIRIQES PGKVAAGRLP RSKDAILLAD LVDSCKPGDE IELTGIYHNN YDGSLNTANG FPVFATVILA NHVAKKDNKV AVGELTDEDV KMITSLSKDQ QIGEKIFASI APSIYGHEDI KRGLALALFG GEPKNPGGKH KVRGDINVLL CGDPGTAKSQ FLKYIEKVSS RAIFTTGQGA SAVGLTAYVQ RHPVSREWTL EAGALVLADR GVCLIDEFDK MNDQDRTSIH EAMEQQSISI SKAGIVTSLQ ARCTVIAAAN PIGGRYDPSL TFSENVDLTE PIISRFDILC VVRDTVDPVQ DEMLARFVVG SHVRHHPSNK EEEGLANGSA AEPAMPNTYG VEPLPQEVLK KYIIYAKERV HPKLNQMDQD KVAKMYSDLR KESMATGSIP ITVRHIESMI RMAEAHARIH LRDYVIEDDV NMAIRVMLES FIDTQKFSVM RSMRKTFARY LSFRRDNNEL LLFILKQLVA EQVTYQRNRF GAQQDTIEVP EKDLVDKARQ INIHNLSAFY DSELFRMNKF SHDLKRKMIL QQF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Reference: A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.Todorov I.T., Pepperkok R., Philipova R.N., Kearsey S.E., Ansorge W., Werner D.J. Cell Sci. 107:253-265(1994). Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.