ATP-dependent translocase ABCB1 (ABCB1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 350-710aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: PSIEAFANAR GAAYEIFKII DNKPSIDSYS KSGHKPDNIK GNLEFRNVHF SYPSRKEVKI LKGLNLKVQS GQTVALVGNS GCGKSTTVQL MQRLYDPTEG MVSVDGQDIR TINVRFLREI IGVVSQEPVL FATTIAENIR YGRENVTMDE IEKAVKEANA YDFIMKLPHK FDTLVGERGA QLSGGQKQRI AIARALVRNP KILLLDEATS ALDTESEAVV QVALDKARKG RTTIVIAHRL STVRNADVIA GFDDGVIVEK GNHDELMKEK GIYFKLVTMQ TAGNEVELEN AADESKSEID ALEMSSNDSR SSLIRKRSTR RSVRGSQAQD RKLSTKEALD ESIPPVSFWR IMKLNLTEWP Y. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Translocates drugs and phospholipids across the membrane . Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins . Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells . Reference: "MDR1 P-glycoprotein is a lipid translocase of broad specificity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine." van Helvoort A., Smith A.J., Sprong H., Fritzsche I., Schinkel A.H., Borst P., van Meer G. Cell 87:507-517(1996). Function: nan