Angiotensin-converting enzyme (ACE2), partial (Active), Macaca fascicularis, recombinant

Organism: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey). Source: Mammalian cell. Expression Region: 18-741aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGE KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPNN PQECLLLDPG LNEIMEKSLD YNERLWAWEG WRSEVGKQLR PLYEEYVVLK NEMARANHYK DYGDYWRGNY EVNGVDGYDY NRDQLIEDVE RTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PTGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VNQAWNAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKVVCHPT AWDLGKGDFR IIMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLLNM LKLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRTYFLE IKHQTILFGE EDVRVADLKP RISFNFYVTA PKNVSDIIPR TEVEEAIRIS RSRINDAFRL NDNSLEFLGI QTTLAPPYQS PVTT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 µg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: angiotensin I + H2O = angiotensin-(1-9) + L-leucine. Reference: n/a. Function: nan