Aldehyde dehydrogenase X, mitochondrial (ALDH1B1),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 167-264aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-GST-tagged and C-terminal V5-Myc-tagged. Target Protein Sequence: SRVISQPDQT LEILMSELDP AVMDQFYMKD GVTAKDVTRE SGIRDLIPGS VIDATMFNPC GYSMNGMKSD GTYWTIHITP EPEFSYVSFE TNLSQTSY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. Reference: "Aldehyde dehydrogenase 1B1: molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme." Stagos D., Chen Y., Brocker C., Donald E., Jackson B.C., Orlicky D.J., Thompson D.C., Vasiliou V. Drug Metab Dispos 38:1679-1687(2010). Function: nan