5'-Nucleotidase (NT5E), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 28-547aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: ELTILHTNDV HSRLEQTSED SSKCVNASRC MGGVARLFTK VQQIRRAEPN VLLLDAGDQY QGTIWFTVYK GAEVAHFMNA LRYDAMALGN HEFDNGVEGL IEPLLKEAKF PILSANIKAK GPLASQISGL YLPYKVLPVG DEVVGIVGYT SKETPFLSNP GTNLVFEDEI TALQPEVDKL KTLNVNKIIA LGHSGFEMDK LIAQKVRGVD VVVGGHSNTF LYTGNPPSKE VPAGKYPFIV TSDDGRKVPV VQAYAFGKYL GYLKIEFDER GNVISSHGNP ILLNSSIPED PSIKADINKW RIKLDNYSTQ ELGKTIVYLD GSSQSCRFRE CNMGNLICDA MINNNLRHTD EMFWNHVSMC ILNGGGIRSP IDERNNGTIT WENLAAVLPF GGTFDLVQLK GSTLKKAFEH SVHRYGQSTG EFLQVGGIHV VYDLSRKPGD RVVKLDVLCT KCRVPSYDPL KMDEVYKVIL PNFLANGGDG FQMIKDELLR HDSGDQDINV VSTYISKMKV IYPAVEGRIK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes Extracellular domain nucleotides into mbrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. Reference: Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y.Eur. J. Biochem. 191:563-569(1990). Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.