IL-10, multi-species recombinant protein

The Multi-Species IL-10 yeast-derived recombinant protein is not tagged and is naturally endotoxin-free. The purity is greater than 98% as visualized by SDS-PAGE analysis. Multi-Species IL-10 applications are for cell culture, ELISA standard, and Western Blot Control. Multi-Species IL-10 yeast-derived recombinant protein can be purchased in multiple sizes. Multi-Species IL-10 Specifications: (Molecular Weight: 18.4 kDa) (Amino Acid Sequence: SRDASTLSDS SCTHFPASLP HMLRELRAAF GKVKTFFQMK DQLNSMLLTQ SLLDDFKGYL GCQALSEMIQ FYLEEVMPQA ENHGPDIKEH VNSLGEKLKT LRLRLHRCHR FLPCENKSKA VEQVKRVFNM LQERGVYKAM SEFDIFINYI ESYMTTKM (158)) (Gene ID: 443342). For research use only. Made in the USA. Protein function: Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. [The UniProt Consortium]