TET3 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC, A

Length: 2814 Sequence: atggaggagc ggtatggaga gaaggggaaa gccatccgga tcgagaaggt catctacacg gggaaggagg gaaagagctc ccgcggttgc cccattgcaa agtgggtgat ccgcaggcac acgctggagg agaagctact ctgcctggtg cggcaccggg caggccacca ctgccagaac gctgtgatcg tcatcctcat cctggcctgg gagggcattc cccgtagcct cggagacacc ctctaccagg agctcaccga caccctccgg aagtatggga accccaccag ccggagatgc ggcctcaacg atgaccggac ctgcgcttgc caaggcaaag accccaacac ctgtggtgcc tccttctcct ttggttgttc ctggagcatg tacttcaacg gctgcaagta tgctcggagc aagacacctc gcaagttccg cctcgcaggg gacaatccca aagaggaaga agtgctccgg aagagtttcc aggacctggc caccgaagtc gctcccctgt acaagcgact ggcccctcag gcctatcaga accaggtgac caacgaggaa atagcgattg actgccgtct ggggctgaag gaaggacggc ccttcgcggg ggtcacggcc tgcatggact tctgtgccca cgcccacaag gaccagcata acctctacaa tgggtgcacc gtggtctgca ccctgaccaa ggaagacaat cgctgcgtgg gcaagattcc cgaggatgag cagctgcatg ttctccccct gtacaagatg gccaacacgg atgagtttgg tagcgaggag aaccagaatg caaaggtggg cagcggagcc atccaggtgc tcaccgcctt cccccgcgag gtccgacgcc tgcccgagcc tgccaagtcc tgccgccagc ggcagctgga agccagaaag gcagcagccg agaagaagaa gattcagaag gagaagctga gcactccgga gaagatcaag caggaggccc tggagctggc gggcattacg tcggacccag gcctgtctct gaagggtgga ttgtcccagc aaggcctgaa gccctccctc aaggtggagc cgcagaacca cttcagctcc ttcaagtaca gcggcaacgc ggtggtggag agctactcgg tgctgggcaa ctgccggccc tccgaccctt acagcatgaa cagcgtgtac tcctaccact cctactatgc acagcccagc ctgacctccg tcaatggctt ccactccaag tacgctctcc cgtcttttag ctactatggc tttccatcca gcaaccccgt cttcccctct cagttcctgg gtcctggtgc ctgggggcat agtggcagca gtggcagttt tgagaagaag ccagacctcc acgctctgca caacagcctg agcccggcct acggtggtgc tgagtttgcc gagctgccca gccaggctgt tcccacagac gcccaccacc ccactcctca ccaccagcag cctgcgtacc caggccccaa ggagtatctg cttcccaagg cccccctact ccactcagtg tccagggacc cctccccctt tgcccagagc tccaactgct acaacagatc catcaagcaa gagccagtag acccgctgac ccaggctgag cctgtgccca gagacgctgg caagatgggc aagacacctc tgtccgaggt gtctcagaat ggaggaccca gtcacctttg gggacagtac tcaggaggcc caagcatgtc ccccaagagg actaacggtg tgggtggcag ctggggtgtg ttctcgtctg gggagagtcc tgccatcgtc cctgacaagc tcagttcctt tggggccagc tgcctggccc cttcccactt cacagatggc cagtgggggc tgttccccgg tgaggggcag caggcagctt cccactctgg aggacggctg cgaggcaaac cgtggagccc ctgcaagttt gggaacagca cctcggcctt ggctgggccc agcctgactg agaagccgtg ggcgctgggg gcaggggatt tcaactcggc cctgaaaggt agtcctgggt tccaagacaa gctgtggaac cccatgaaag gagaggaggg caggattcca gccgcagggg ccagccagct ggacagggcc tggcagtcct ttggtctgcc cctgggatcc agcgagaagc tgtttggggc tctgaagtca gaggagaagc tgtgggaccc cttcagcctg gaggaggggc cggctgagga gccccccagc aagggagcgg tgaaggagga gaagggcggt ggtggtgcgg aggaggaaga ggaggagctg tggtcggaca gtgaacacaa cttcctggac gagaacatcg gcggcgtggc cgtggcccca gcccacggct ccatcctcat cgagtgtgcc cggcgggagc tgcacgccac cacgccgctt aagaagccca accgctgcca ccccacccgc atctcgctgg tcttctacca gcacaagaac ctcaaccagc ccaaccacgg gctggccctc tgggaagcca agatgaagca gctggcggag agggcacggg cacggcagga ggaggctgcc cggctgggcc tgggccagca ggaggccaag ctctacggga agaagcgcaa gtgggggggc actgtggttg ctgagcccca gcagaaagag aagaaggggg tcgtccccac ccggcaggca ctggctgtgc ccacagactc ggcggtcacc gtgtcctcct atgcctacac gaaggtcact ggcccctaca gccgctggat Protein function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl- CpG (PubMed:29276034). [The UniProt Consortium]