Rhodamine 123 (chloride)

Rhodamine 123 is a membrane-permeable cationic dye that is readily accumulated within living cells. It is a substrate for the efflux pump P-glycoprotein (P-gp, also known as multidrug resistance protein 1 and ABCB1) and is rapidly exported from cells with functional P-gp. As P-gp is expressed on a population of stem cells known as the side population, rhodamine 123 is used to detect this group of stem cells. Rhodamine 123 also accumulates within mitochondria due to its positive charge and can inhibit oxidative phosphorylation. Rhodamine 123 has excitation/emission maxima of 507/529 nm.Formal Name: 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-xanthylium, monochloride. CAS Number: 62669-70-9. Synonyms: R-22420, R-302, RH123. Molecular Formula: C21H17N2O3 . Cl. Formula Weight: 380.8. Purity: >95%. Emission: 529 nm. Excitation: 507 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: 0.1 M HCl: 10 mg/ml, DMF: 10 mg/ml, DMSO: 10 mg/ml, Ethanol: 10 mg/ml. SMILES: NC1=CC2=[O+]C3=C(C(C4=CC=CC=C4C(OC)=O)=C2C=C1)C=CC(N)=C3.[Cl-]. InChi Code: InChI=1S/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20,/h2-11H,22-23H2,1H3,1H/q+1,/p-1. InChi Key: TUFFYSFVSYUHPA-UHFFFAOYSA-M.