U-0126

U-0126 is a MEK inhibitor with IC50 values of 72 nM and 58 nM for MEK1 and MEK2, respectively. It is noncompetitive with respect adenosine triphosphate (ATP) and its phosphorylation target ERK and it shows little to no inhibition against a number of other kinases including PKC, Ab1, Raf, MEKK, ERK, JNK, MKK-3, MKK-4, MKK-6, Cdk2, and Cdk4. However, U-0126 does phosphorylate and activate AMP-activated protein kinase (AMPK) in a dose-dependent manner (EC50 = 15 µM in HEK293 cells). It increases the ratios of ADP to ATP and AMP to ATP and increases phosphorylation of the AMPK target acetyl-CoA carboxylase (ACC).Formal Name: 2,3-bis[amino[(2-aminophenyl)thio]methylene]-butanedinitrile. CAS Number: 109511-58-2. Molecular Formula: C18H16N6S2. Formula Weight: 380.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF: PBS (pH 7.2)(1:2): 0.3 mg/ml, DMSO: 25 mg/ml, Ethanol: 0.5 mg/ml. lambdamax: 247, 308 nm. SMILES: N/C(SC1=CC=CC=C1N)=C(C#N)\C(C#N)=C(N)\SC2=C(N)C=CC=C2. InChi Code: InChI=1S/C18H16N6S2/c19-9-11(17(23)25-15-7-3-1-5-13(15)21)12(10-20)18(24)26-16-8-4-2-6-14(16)22/h1-8H,21-24H2/b17-11+,18-12+. InChi Key: DVEXZJFMOKTQEZ-JYFOCSDGSA-N.