Ac-RLR-AMC (trifluoroacetate salt)

Ac-RLR-AMC is a fluorogenic substrate for the 26S proteasome. Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify the trypsin-like activity of 26S proteasomes. AMC displays excitation/emission maxima of 380/440-460 nm, respectively.Formal Name: (S)-2-acetamido-5-guanidino-N-((S)-1-(((S)-5-guanidino-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopentan-2-yl)amino)-4-methyl-1-oxopentan-2-yl)pentanamide, 2,2,2-trifluoroacetate salt. Synonyms: Ac-Arg-Leu-Arg-AMC, Ac-Arg-Leu-Arg-7-amino-4-Methylcoumarin. Molecular Formula: C30H46N10O6 . XCF3COOH. Formula Weight: 642.8. Purity: >98%. Emission: 440-460 nm. Excitation: 380 nm. Formulation: (Request formulation change), A lyophilized solid. Solubility: Acetic Acid (5%): soluble. lambdamax: 231, 296, 328 nm. SMILES: CC(C1=CC=C(NC([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC(C)=O)CCCNC(N)=N)=O)=O)CCCNC(N)=N)=O)C=C1O2)=CC2=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C30H46N10O6.C2HF3O2/c1-16(2)13-23(40-27(44)21(37-18(4)41)7-5-11-35-29(31)32)28(45)39-22(8-6-12-36-30(33)34)26(43)38-19-9-10-20-17(3)14-25(42)46-24(20)15-19,3-2(4,5)1(6)7/h9-10,14-16,21-23H,5-8,11-13H2,1-4H3,(H,37,41)(H,38,43)(H,39,45)(H,40,44)(H4,31,32,35)(H4,33,34,36),(H,6,7)/t21-,22-,23-,/m0./s1. InChi Key: CEEMPRHZPRGAAK-RGRVRPFLSA-N.