Anti-Retinol Binding Protein 4 (RBP4, PRBP, PRO2222)

RBP4, also known as plasma retinol-binding protein, is a member of the lipocalin superfamily that solubilizes and stabilizes retinol (vitamin A) for transport to the peripheral tissues. RBP4 shows retinol-dependent secretion by the liver, and its deficiency causes night blindness. In plasma, the 183aa RBP4-retinol complexes with transthyretin (TTR), previously known as thyroxine-binding prealbumin, which prevents loss during glomerular filtration. Human and mouse RBP4 share 86% aa identity. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2-8ug/ml , Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.