Anti-Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensit

PDX-1 (Pancreas-duodenum homeobox 1, also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42kD member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta-cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta-cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284aa in length (Accession # P52946). It contains one transactivation domain (aa13-73) a PBX1 P-P-M-W-K dimerization motif (aa119-124), and a DNA-binding homeodomain with an embedded NLS (aa148-206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively. Applications: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Immunocytochemistry: 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.