Anti-BIRC3 (IAP2, cellular, cIAP2, Inhibitor of Apoptosis 2, API2, Baculoviral IAP Repeat-containing

Apoptosis inhibitor 2 (API2) is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. API2 inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The amino acid sequence predicts three baculovirus IAP repeat domains and a ring finger domain. The names of API1 and API2 have been confusing. Suffice it to say that 'apoptosis inhibitor 2' is hiap-1 (inhibitor of apoptosis 1). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:3000, Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.