LipidLaunch(TM) SM-102 LNP (mCherry)

LipidLaunch(TM) SM-102 LNP (mCherry) is a solution containing lipid nanoparticles (LNPs) composed of the ionizable cationic amino lipid SM-102 (Cay-33474), cholesterol (Cay-9003100), the phospholipid 1,2-distearoyl-sn-glycero-3-PC (Cay-15100, Cay-39189), and the lipid excipient DMG-PEG(2000) (Cay-33945) at a molar ratio of 50:38.5:10:1.5 and encapsulating mRNA encoding the fluorescent protein mCherry. It is intended for proof-of-concept experiments to determine whether SM-102-based LNPs can effectively lead to expression of a protein of interest in a target cell type, either in vitro or in vivo. mCherry has excitation/emission maxima of 587/610 nm, respectively.Suggested in vitro use: Thaw LNPs on ice with occasional gentle swirling (do not vortex). Using a gentle pipetting technique, dilute 1:100-1:500 in complete cell culture media (with serum) and add to subconfluent adherent cells in a fluorescence imaging-compatible tissue culture plate. Expression of mCherry may be detectable as early as six hours after treatment. Optimal conditions are highly dependent on cell type.Emission: 610 nm. Excitation: 587 nm. Formulation: (Request formulation change), TBS, pH 7.5, with 10% sucrose.