XGAL, 40mg/ml in DMF (X-Gal) (5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (galactopyranoside) in D

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Synonyms: X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside), BCIG, CAS: 7240-90-6, 68-12-2, Molecular Formula: C14H15BrClNO6DMF, Molecular Weight: 408.61, Purity (HPLC): >99%, Concentration: ~40mg/ml, Form: Supplied as a liquid in dimethylformamide. Solubility: Colorless to pale yellow, clear, Identity by I.R. or NMR: Conforms to structure, RNase, DNase or Proteases: None Detected, Quality Control (Blue-White Assay): [LacZ+]: Pale blue center/dense blue periphery, [LacZ-]: Faint blue center/white periphery, Storage and Stability: Store at -20°C. Aliquots are stable for 12 months after receipt. Protect from light. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards:  All products should be handled by qualified personnel only, trained in laboratory procedures. Also Available: X1000: XGAL