Gelite(TM) X100 DNA Gel Stain *10,000X Water Solution*

Excitation: 513 nm Emission: 552 nm Absorbance: 509 nm. AAT Bioquest is committed to designing our products to be environment friendly. It's part of how we enable our customers to make the world healthier, cleaner, and safer. Ethidium bromide (EtBr) has been commonly used as a DNA stain for many years. However, EtBr is harmful if swallowed, and is very toxic if inhaled. EtBr has been shown to be mutagenic in various tests and is an aquatic toxin. SYBR(R) Safe was introduced as a safer alternative to EtBr and SYBR(R) Green, but unfortunately it is much less sensitive than SYBR(R) Green. It only has sensitivity comparable to EtBr. Gelite(TM) X100 has been developed specifically to be less hazardous than EtBr for staining DNA in agarose and acrylamide gels with much higher sensitivity. Gelite(TM) X100 has greatly improved safety and uncompromised sensitivity. The exceptional sensitivity and strong DNA binding affinity of Gelite(TM) X100 allows for DNA to be stained prior to or post electrophoresis without destaining. In addition to its superior binding properties, Gelite(TM) X100 is essentially non-fluorescent in the absence of nucleic acids showing very low background fluorescence. Upon binding to nucleic acids, Gelite(TM) X100 exhibits a large fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite(TM) X100 was optimized to be compatible with a variety of instruments including UV and blue-light transiluminators, gel documentation systems and laser scanners. It is the first single formulation that can be used either in green or red channel at your preference. Unlike the membrane-permeant SYBR(R) Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite(TM) X100 make it a much safer and noncytotoxic alternative. Furthermore, Ames testing has confirmed Gelite(TM) X100 to be significantly less mutagenic than EtBr and SYBR(R) Green, even at concentrations well above the working concentration used for gel staining. Ames mutagenicity test was performed in a dose dependent manner for all test dyes pretreated with a S9 fraction from liver (SYBR(R) is a trademark of ThermoFisher).