Rabbit TrueBlot® Western Blot Kit

The Rabbit TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Rabbit IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced & denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-rabbit IgG reagent). Rabbit IgG TrueBlot® is the unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-rabbit IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Rabbit IgGTrueBlot®. Rabbit IgG TrueBlot is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.