ReadiLink(TM) Rapid mFluor(TM) Violet 610 Antibody Labeling Kit *Microscale Optimized for Labeling 5

Absorbance (nm): 594. Correction Factor (260 nm): 0.532. Correction Factor (280 nm): 0.66. Extinction coefficient (cm^-1 M^-1): 90000 (PBS). Excitation (nm): 421. Emission (nm): 612. Quantum yield: 0.3 (PBS). ReadiLink(TM) Rapid mFluor(TM) Violet 610 Antibody Labeling Kits provide a quick and convenient method to label antibodies, proteins (>10 kDa), or other amine-containing biomolecules. Leveraging a unique conjugation chemistry, ReadiLink(TM) Antibody Labeling Kits enables researchers to label microscale volumes of antibodies in two easy steps, with no purification needed and 100% recovery of the labeled product. Each kit includes sufficient mFluor(TM) Violet 610 dye to facilitate two distinct labeling reactions of 50 µg antibody samples. The resulting conjugates are ideal for a wide range of applications, including flow cytometry, fluorescent microscopy techniques, immunocytochemistry (ICC), immunohistochemistry (IHC), ELISA, and indirect FISH. Compared to Alexa Fluor dyes and conventional dye counterparts, mFluor(TM) dyes are brighter, more photostable, and resistance to changes in pH between pH 4 to 10. The mFluor(TM) Violet 610 dyes are optimally excited by violet lasers and emit red fluorescence ~610 nm. These properties make them well-suited for spectral fluorescence flow cytometry, providing researchers with a tool for detailed biological analysis with unparalleled precision and sensitivity. With ReadiLink(TM) Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.