Buccutite(TM) Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody

Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC. One end of the SMCC reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. AAT Bioquest has developed a convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: BuccutiteT MTA and BuccutiteT FOL. MTA is added to one protein, while FOL is added to another protein. Protein-protein cross-linking reaction is initiated by mixing Protein-1-Buccutite T MTA and Protein-2-Buccutite T FOL. This crosslinking reaction occurs under extremely mild neutral conditions without any catalyst required, and it is robust and efficient.