Buccutite(TM) Poly-HRP Antibody Conjugation Kit

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level. Buccutite(TM) Poly-HRP Antibody Conjugation Kit is designed for rapid preparation of poly-HRP conjugated antibodies. The kit provides the poly-HRP that is pre-activated with our proprietary linker Buccutite(TM) FOL. A targeted antibody is activated with Buccutite(TM) MTA (provided in the kit) to give MTA-activated antibody. The simple mix of MTA-activated antibody with FOL-activated poly-HRP to give the desired poly-HRP-labeled antibody conjugate. The reaction occurs under extremely mild conditions without a catalyst required. The poly-HRP antibody conjugate prepared with Buccutite(TM) Poly-HRP Antibody Conjugation Kit is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, western blotting, immunohistochemistry (IHC) assays. It can also be used with TSA or our Styramide(TM) fluorescent HRP substrates for ultrasensitive detection of low abundant biological targets.