NZY Total RNA isolation kit

NZY Total RNA Isolation kit is designed for the easy purification of total RNA of highest integrity (longer than 200 bases) from a variety of sources such as animal tissues, bacteria cells and cell cultures. This method uses a denaturing lysis buffer containing guanidine thiocyanate, which inactivates cellular RNases, to ensure the recovery of intact RNA molecules. Ethanol is added to provide selective binding of total RNA into the silica membrane column and impurities are efficiently washed away. To prevent the contamination with DNA, a DNase I solution is directly added onto the silica membrane of the binding column. High-quality RNA is then eluted in RNase-free water. Components: * Buffer NR * Buffer NI * Buffer NRW1 * Buffer NRW2 (concentrated) * RNase-free water * Digestion buffer * DNase I (lyophilized) * NZYSpin Homogenization Columns (purple rings) * NZYSpin Binding Columns (blue rings) * Collection tubes (1.5 mL and 2 mL). Shipping Conditions: Room Temperature. Storage Conditions: 15 °C to 25 °C. Application: cDNA synthesis, RT-PCR, RT-qPCR. Starting Material: Animal Tissue, Cultured Cells, Gram-negative bacteria, Gram-positive bacteria, Yeast. Scale: Miniprep. Features: DNA removal, Manual. Format: Column. Product Category: RNA Purification. Isolation Technology: Silica membrane. Protocol Time: Approx. 30 min