Sonic hedgehog protein (Shh), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.Coli. Expression Region: 26-198aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: GPGRGFGKRR HPKKLTPLAY KQFIPNVAEK TLGASGRYEG KITRNSERFK ELTPNYNPDI IFKDEENTGA DRLMTQRCKD KLNALAISVM NQWPGVKLRV TEGWDEDGHH SEESLHYEGR AVDITTSDRD RSKYGMLARL AVEAGFDWVY YESKAHIHCS VKAENSVAAK SGG. Purity: >95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 µg/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269, PubMed:14687547, ECO:0000269, PubMed:7736596}. Reference: n/a. Function: Sonic hedgehog protein