Osteopontin (Spp1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 17-290aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LPVKVTDSGS SEEKLYSLHP DPIATWLVPD PSQKQNLLAP QNAVSSEEKD DFKQETLPSN SNESHDHMDD DDDDDDDDGD HAESEDSVDS DESDESHHSD ESDETVTAST QADTFTPIVP TVDVPNGRGD SLAYGLRSKS RSFQVSDEQY PDATDEDLTS HMKSGESKES LDVIPVAQLL SMPSDQDNNG KGSHESSQLD EPSLETHRLE HSKESQESAD QSDVIDSQAS SKASLEHQSH KFHSHKDKLV LDPKSKEDDR YLKFRISHEL ESSS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. Reference: Osteopontin, a transformation-associated cell adhesion phosphoprotein, is induced by 12-O-tetradecanoylphorbol 13-acetate in mouse epidermis.Craig A.M., Smith J.H., Denhardt D.T.J. Biol. Chem. 264:9682-9689(1989). Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction., FUNCTION